rabbit anti 4 hydroxynonenal 4hne  (Bioss)

Journal: Frontiers in Aging Neuroscience

Article Title: Ketogenic interventions enhance REM sleep in females and support memory in aged rats

doi: 10.3389/fnagi.2026.1797686

Figure Lengend Snippet: Effect of ketogenic diet intervention on oxidative stress and neuroinflammation in aged rats. (A) Representative immunofluorescence images showing β -III tubulin for neurons (red), Iba1 for microglia (cyan), GFAP for astrocytes (blue), and 4HNE (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fifth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. All images were acquired at 20X magnification and 3×2 tiles. Graphs showing the average intensity of 4HNE in neurons (B) , microglia (C) , and astrocytes (D) , respectively, quantified using the Ilastik-QuPath workflow for calculating the mean 4HNE intensity in each cell type expressed as arbitrary units (A.U.). Graphs showing the integrated density (Int. Den.) per mm 2 expressed as arbitrary units (A.U.) (E) and no. of 4HNE puncta per mm 2 (F) quantified using NIH-ImageJ software. (G) Representative immunofluorescence images showing β-III tubulin (red), Iba1 (cyan), and TREM2 (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fourth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. Graphs of mean TREM2 intensity in neurons (H) and microglia (I) , respectively quantified using the Ilastik-QuPath workflow for quantification of mean TREM2 intensity in each cell type expressed as A.U. (J) TREM2 integrated density per mm2 expressed as arbitrary units (A.U.) quantified using NIH-ImageJ software. Individual data points represent data from the CA1 region of the hippocampus from one brain tissue section; three brain sections were imaged per rat and approximately 100–200 cells were analyzed per cell type. The number of detections for each individual cell type for each marker is given in , and the number of rats used for the analysis are given in . Data represent Mean ± SEM. Mixed-effects two-way ANOVA (sex × diet) with animal as a random factor (fields nested within animals) and Tukey post hoc test. * p < 00.05, ** p < 0.01. Control diet (CD) and ketogenic diet (KD).

Article Snippet: The following primary antibodies were used: rabbit anti- 4-hydroxynonenal (4HNE) (1:500; Bioss bs-6313R), rabbit anti- 3-nitrotyrosine (3-NT) (1:200; Bioss BS-8551R), rabbit anti- triggering receptor expressed on myeloid cells 2 (TREM2) (1:200; Bioss BS-2723R), rabbit anti- NLR family pyrin domain containing 3 (NLRP3) (1:100; NBP2-12446), mouse anti-beta- III tubulin (1:400; Abcam AB78078), rat anti- ionized calcium-binding adaptor molecule 1 (IBA) (1:1000; Abcam AB283346 ), chicken anti- glial fibrillary acidic protein (GFAP) (1:1000; Abcam AB4674).

Techniques: Immunofluorescence, Software, Marker, Control

Journal: Experimental Physiology

Article Title: A single bout of whole-leg, peristaltic pulse external pneumatic compression upregulatesPGC-1αmRNA and endothelial nitric oxide sythase protein in human skeletal muscle tissue

doi: 10.1113/ep085160

Figure Lengend Snippet: Figure 3. The acute effect of EPC on 1 and 4 h post-treatment skeletal muscle superoxide dismutase 2 (SOD2) mRNA (A), SOD2 protein content (B) and 4-hydroxynonenal (4HNE) protein levels (D); a representative image of SOD2 and 4HNE protein levels and respective Ponceau images are presented in C and E, respectively All data are expressed as the fold change from PRE levels (mean ± SEM, n = 7–10 subjects per target for mRNA data and n = 7 for 4HNE protein levels). Dotted lines represent individual response patterns. Significance from between-time points comparisons using Student’s paired t tests are indicated within each panel.

Article Snippet: Rabbit anti-4-hydroxynonenal (4HNE) IgG (1:1000 dilution; Abcam, Cambridge, MA, USA), anti-eNOS IgG (1:500 dilution; Cell Signaling), anti-PGC-1α (1:1000 dilution; Santa Cruz, Dallas, TX, USA), anti-HIF-1α (1:1000 dilution; Santa Cruz) and anti-SOD2 (1:1000 dilution; Santa Cruz) were incubated with membranes overnight at 4°C in 5% bovine serum albumin.

Techniques: